Transferrin, a commonly used marker of the endocytic recycling pathway, binds to cell surface receptors that are internalized via clathrin-coated vesicles in a Rab5-dependent manner to form the EE (Bucci et al., 1992). Snx3 Regulates Recycling of the Transferrin Receptor and Iron Assimilation The Harvard community has made this article openly available. REACTOME_TRANSFERRIN_ENDOCYTOSIS_AND_RECYCLING The CD71/transferrin system is famous for iron-transport into proliferating cells and is a prototypic endocytic receptor, which is recycling to the cell surface. The role of SNAREs in transferrin recycling has been studied previously, but the the overall picture is not clear. Starve cells for 30 mins at 37˚C in pre-warmed serum-free medium supplemented SNX3 Regulates Recycling of the Transferrin Receptor and ... In addition to these effects in vivo, wortmannin is a potent inhibitor (IC50 -15 nM) of a cell-free assay that detects the delivery of endocytosed probes into a commoncompartment. [PDF] Studies of Transferrin Recycling Reconstituted in ... Thus, each receptor must recycle a ligand, on the average, each 15.8 min. Read more related scholarly scientific articles and abstracts. Read "Evidence for a pool of non‐recycling transferrin receptors in peripheral sheep reticulocytes, Journal of Cellular Physiology" on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. The recycling ligand transferrin was also internalized and enriched in the Rab3b/3c-positive vesicles. Endocytosis and recycling of membrane proteins are key processes for nutrient uptake, receptor signaling and synaptic transmission. The rate of transferrin recycling in thioglycollate-elicited murine peritoneal macrophages (thio-macrophages) was exceedingly rapid, with exocytic rate constants that were 2- to 3-fold higher than those of most other cells. Moreover, the loss of Rab3b/3c by siRNA silencing reduced the fast phase recycling rates of transferrin receptors. Transferrin and transferrin receptor (TfR) regulate iron uptake in almost all cell types, and are frequently used to study endocytosis and recycling. rabllBP did not affect transferrin recycling, overexpression of a truncated form of the protein, rabllBP(1-504), that includes the rabll-binding site but lacks the WD40 domains inhibited recycling as strongly as does a dominant negative rabll mutant protein that does not bind GTP. TBC1D16 is a Rab4A GTPase activating protein that ... Department of Biology, Massachusetts Institute of Technology, Boston, Massachusetts 02139. Production or exocytosis (E1-E4) was measured in the current study. After it has bound to its surface receptors, transferrin enters cells through a process of receptor-mediated endocytosis and deposits iron intracellularly. Strikingly, the inhibition caused by the truncated rabllBP was . Please share how this access benefits you. Expression of a dominant-negative mutation of an ERC-associated protein, mRme-1 (G429R), results in the slowing of both DHE and Tf receptor return to the . CiteSeerX - Scientific documents that cite the following paper: The End2 mutation in CHO cells slows the exit of transferrin receptors from the recycling compartment but bulk membrane recycling is unaffected. Most of the transferrin is recycled back to the cell surface via the slow recycling pathway through the ERC in a process that . The endocytosis of transferrin by rabbit reticulocytes was 2- to 2.5-times faster than guinea-pig reticulocytes. We wondered whether transferrin recycling is altered in these cells. The sorting mechanisms responsible for earlier trafficking steps in intracellular transferrin cycle, however, are poorly understood. The small GTPase Rab5 regulates the early endocytic pathway of transferrin (Tfn), and Rab5 deactivation is required for Tfn recycling. Because the GTPase Rab11 has been implicated in transferrin recycling in other cells, we determined its role in . Your story matters Citation Chen, Caiyong, Daniel Garcia-Santos, Yuichi Ishikawa, Alexandra Seguin, Liangtao Li, Katherine H. Fegan, Gordon J. Hildick-Smith, et al. The transferrin receptor (TfR) undergoes multiple rounds of clathrin-mediated endocytosis and reemergence at the cell surface, importing iron-loaded transferrin (Tf) and recycling apotransferrin after discharge of iron in the endosome. Brief description. (A) Timecourse of transferrin uptake in reconstituted and knockout cells. Whereas, the effect of human HFE (hHFE) on transferrin/ transferrin receptor association, as well as on transferrin receptor recycling and the level of cellular iron pools in various cell lines was analyzed, very little is known about the mouse HFE (mHFE) protein. Full text 1983 Aug 25; 258 (16):9681-9689. Expression of the wild-type protein and a GTP hydrolysis-deficient mutant led to the redistribution of transferrin receptor to large cRab22a-positive structures in the periphery of the cell and to a significant decrease in the plasma membrane receptor. We wondered whether transferrin recycling is altered in these cells. Immunoprecipitation of syntaxin 13 revealed that, in Triton X-100 extracts, syntaxin 13 is present in a complex(es) comprised of SNAP, VAMP 2/3, and SNAP-25. Elevated MACC1 expression caused also the activation and internalization of EGFR, a higher rate of receptor recycling, as well as earlier and stronger receptor activation and downstream signaling. be in recycling endosomes nearby and that the TGN pool of mATG9 may be quite small. Surface-bound transferrin is rapidly endocytosed (t1/2 = 3.5 min). Furthermore, the Rab3b/3c-positive compartments were colocalized with a fraction of Rab27a at a juxtaposition of phagosomes. Increasing evidence suggests that PtdIns[4,5]P 2 is directly involved in the regulation of trafficking steps to and from the plasma membrane, although the precise steps relying on proper PtdIns[4,5]P 2 balance remain to be identified.. To test the role of PtdIns[4,5]P 2 in endocytosis, we first performed . 1995 Academic Article GET IT Times cited: 32; An internalization motif is created in the cytoplasmic domain of the transferrin receptor by substitution of a tyrosine at the first position of a . Developmentally regulated GTP-binding protein 2 coordinates Rab5 activity and transferrin recycling. IgG concentration reached 1.1 mg ml −1 in a month of recycling and its ratio to the total protein was 45.8%. Rab11 is a small GTP-binding protein that in cultured mammalian cells has been shown to be concentrated in the pericentriolar endosomal recycling compartment and to play a key role in passage of the recycling transferrin receptor through that compartment [Ullrich, O., Reinsch, S., Urbé, S., Zerial, M. & Parton, R. G. (1996) J. Molecular biology of the cell. At pH 5.0 both apo- and diferric Tf bind with high affinity; at pH 7.4 diferric Tf binds avidly, but apoTf binds very poorly. This is most simply explained by assuming that recycling of receptors back to the cell surface is inhibited as cells enter mitosis but that internalisation continues for a short while, causing surface receptor depletion. recycling compartment (18). The possibilities that the recycling of the transferrin receptor is a rate-limiting step in the efflux of endocytosed transferrin, and that the receptor functions as a trans-membrane Fe transporter were investigated in untransfected Ltk-cells and in cells transfected with different levels of DNA for wild-type, mutant and chimeric human transferrin receptors. Transferrin endocytosis and recycling. The endosome is not fused with a lysosome but recycles its content back to the cell surface where soon transferrin dissociates from its . The Journal of biological chemistry. At steady state, DHE co-localizes extensively with transferrin (Tf), a marker for the endocytic recycling compartment (ERC), and redistributes with Tf in cells with altered ERC morphology. Images recorded at 2.5, 5, 10, 20 and 30 . Efficient transferrin receptor recycling is reconstituted when donor cytosol and ATP are added to the streptolysin O permeabilized cells. Transferrin endocytosis and recycling. 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